Cell Type-Specific Promoters of Volvox carteri for Molecular Cell Biology Studies

The multicellular green alga Volvox carteri has emerged as a valuable model organism for investigating various aspects of multicellularity and cellular differentiation, photoreception phototaxis, cell division, biogenesis the extracellular matrix morphogenetic movements. While range molecular tools bioinformatics resources have been made available exploring these topics, establishment type-specific promoters in V. not achieved so far. Therefore, here, we conducted thorough screening transcriptome data from RNA sequencing analyses order to identify potential promoters. Eventually, chose two putative strong promoters, with one exhibiting specific expression reproductive cells (gonidia), PCY1 promoter, other somatic cells, PFP promoter. After cloning both promoter regions, they were introduced upstream luciferase reporter gene. By using particle bombardment, DNA constructs stably integrated into genome carteri. results analyses, which at transcript protein levels, demonstrated that drive their respective target types. Transformants considerably diverse levels chimeric genes identifiable. In conclusion, analysis allowed identification Reporter gene actual usability investigated proven be potent genetic engineering